gms | German Medical Science

Objectives/Interrogation: Comparison of two models of arterialized venous flap in minipigs with dissecting the cutaneous vessels in minipigs, in order to provide anatomical foundations for arterialized venous flap research.

Methods: Deep superior epigastric artery (DSEA) of minipig was probed by PeriCam PSI System with Laser Speckle Doppler Perfusion Imaging techniques, and two types of arterialized venous flap models were established on the torso integument of minipigs with anatomical techniques. Twenty healthy minipigs were randomized into two groups with self-control bilateral contrast test method, experimental groups (A group): end to side anastomosis was done between DSEA and cutaneous vein of flap; end to end anastomosis was control groups (B group). The area of the flaps were designed approximately 8cm*6cm. Blood flow perfusion (PU) of all the flaps in two groups were probed by PeriCam PSI System at different times.

Results and Conclusions: According to the measurement data of blood flow perfusion, A groups were 83.62±3.14, and B groups were 98.14±6.54. A groups significantly lower than B groups in early postoperative period (P<0.05), especially within 72 hours, that meant the preload of flap of control groups were higher than experimental groups. This study describes the good results obtained with two types of arterialized venous flap models in minipigs, and shows the significant difference between these two types of models in blood flow perfusion. In conclusion, these operative procedures are suitable for set up arterialized venous flap models in minipigs which can provide anatomical basis for the research of arterialized venous flap.