Artikel
A new immuno-qPCR method for malaria detection
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Veröffentlicht: | 17. Dezember 2014 |
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Gliederung
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The goal of this project is to improve diagnostics for malaria, one of the most deadly infectious diseases. All current methods of detecting Plasmodium show several limitations and disadvantages. Immuno-qPCR uses a DNA-conjugated antibody to detect a specific antigen by PCR amplification. Its advantage is its unprecedented detection sensitivity. However, one major challenge in immuno-qPCR is the synthesis of protein-DNA conjugates. We have adapted and evaluated the universal ultra-sensitive immuno-qPCR diagnostic platform (UUDP) for the quantitative detection of malaria parasites. The UUDP uses a unique self-assembling adapter for antibody-DNA conjugation consisting of the DNA-binding protein Tus fused to protein G. Here we present a new optimized UUDP assay for the detection of pLDH antibodies, which are widely used in commercial diagnostic tests.