Artikel
Oxidative stress in soccer players
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Veröffentlicht: | 24. Oktober 2011 |
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Gliederung
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Objective: The aim of our work was to optimise the oxidative stress (OS) markers in soccer players (SP) during moderate and high intensity exercise.
Material/Methods: In a number of 13 SP (20±3 years old) and 22 control subjects, age matched (medical students). Plasma samples were taken for reactive oxygen metabolites (d-ROMs), lipid peroxidation (LP) and nitric oxide (NO) determination. The d-ROMs test was performed by measurement of hydroperoxide levels (Diacron, Italy) in which transitional metals transfer to peroxide presence in free radical (FR) formation followed by alchilamine trapping. Lipid peroxidation was measured by fluorimetric method by its end product malonyldialdehyde (MDA). For NO determination the method of nitrate enzyme reduction with the Greiss reagent was used (OXIS, USA). The study was performed in two sessions: I - treadmill performance (exposure to moderate exercise); and II - match performance (exposure to high intensity exercise). The blood analysis were taken before treadmill (BT); after treadmill (AT); before match (BM); and after match (AM). Moderate intensity exercise was considered as submaximal exercise treadmill test (50% VO2max). High intensity exercise was considered for the match (80% VO2max).
Results: No significant difference between any of the certain parameters of the same time measurements in SP and control group was found. FR showed increased value in SP from 253±36 UCarr BT to 286±30 UCarr AT (p<0.05). Control subjects showed even more FR production than SP, from 245±20 UCarr BT to 295±25 UCarr AT (p<0.01). The value of FR was more increased AM in SP, 357±49 UCarr and higher LP of 4.5±0.9 μmol/L AM was found in SP (p<0.05). The value of NO in SP increased from 50±18 μmol/L BT up to 98±29 μmol/L AT (p<0.05).
Conclusion: Due to our results we may suggest that moderate-intensity aerobic exercise augments endothelium-dependent vasodilatation through the increased production of NO and that high intensity exercise possibly increases OS. These findings suggest that these OS parameters may be useful markers for better approach and evaluation of the training program in sport medicine.
References
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