Artikel
Detection of fluorescent porphyrins after exposure of pediatric brain tumors to 5-aminolevulinic acid
Suche in Medline nach
Autoren
Veröffentlicht: | 4. Juni 2012 |
---|
Gliederung
Text
Objective: Fluorescence-guided surgery with 5-aminolevulinic acid (5-ALA) makes more complete resections of contrast-enhancing tumors in adults possible. 5-ALA elicits synthesis and accumulation of fluorescent porphyrins in various epithelia and cancerous tissues, which can be visualized using a modified neurosurgical microscope with blue light. Although this technique is well established in adults, it has not been investigated systematically in children even though more complete resections of many pediatric brain tumors are assumed to improve the prognosis. We therefore investigated the feasibility of 5-ALA guided surgery in typical pediatric brain tumors in vitro.
Methods: Cell cultures of medulloblastoma [DAOY and UW228], cPNET [PFSK] rhabdoid tumor [BT1] and ependymoma [RES196] were incubated with 5-ALA for 1, 3, 6, 9, 12 and 24 hours, and the fluorescence intensities of porphyrins were determined using a fluorescence-activated cell sorter (FACS). In addition, we incubated the same cell lines with 5-ALA for only one hour and determined the fluorescence intensity after 1, 3, 6, 9, 12 and 24 hours in order to measure the kinetics of accumulation. C6 and U87-glioma cell lines served as positive controls.
Results: We found strong fluorescence signal in all pediatric brain tumor lines compared to control cells (p<0.05), similar to the signal found in C6 and U87-glioma cells.
Conclusions: We conclude that the exposure of typical pediatric cell tumors to 5-ALA induces accumulation of fluorescent porphyrins in these cells. This indicates that fluorescence-guided surgery of these tumors is feasible. FACS appears to be a reliable method for detecting porphyrin fluorescence in cell cultures.