Artikel
Hypoxia Inducible Factor HIF-3 Gene Expression in Glioblastoma
Hypoxia-inducible-Factor-HIF-3-Genexpression in Glioblastomen
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Autoren
Veröffentlicht: | 23. April 2004 |
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Gliederung
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Objective
Primary and secondary gliobastomas are characterized by different genetic alterations. In order to identify new tumor-associated genes, glioblastoma probes were analyzed using "Human Unigene Set II" filters containing 72.000 cDNA clones representing genes of all human organs.
Methods
RNA was extracted from 74 tumor probes including low-grade astrocytomas and primary and secondary glioblastomas, reverse transcribed into cDNA and hybridized to cDNA filters.
Results
In comparison to regular brain tissue, we identified the hypoxia-inducible factor HIF-3 alpha being overexpressed in 40% of all glioblastomas (23/56) and in 30% of anaplastic astrocytoma (4/14). Samples from low-grade glioma did not show any overexpression.
Conclusions
HIF-3 transcription factor induces gene expression by binding to a hypoxia-responsive element. Enhanced genes might be involved in angiogenesis. It is known that HIF-1 alpha is upregulated in about 70% of glioblastomas. Our findings indicate that HIF-3 is also involved in the neovascularisation of high-grade astrocytoma in response to hypoxia.